TCBS AGAR 500 GRAM HIMEDIA Thiosulfate Citrate Bile Salt Sucrose

Media Thiosulfate Citrate Bile Salt Sucrose (TCBS)
TCBS Agar HImedia M189-500g
Intended Use:
TCBS Agar is recommended for the selective isolation and cultivation of Vibrio cholerae and other enteropathogenic
Vibrio's causing food poisoning from clinical and food specimen.
Composition**
Ingredients Gms / Litre
Proteose peptone 10.000
Yeast extract 5.000
Sodium thiosulphate 10.000
Sodium citrate 10.000
Bile 8.000
Sucrose 20.000
Sodium chloride 10.000
Ferric citrate 1.000
Bromo thymol blue 0.040
Thymol blue 0.040
Agar 15.000
Final pH ( at 25C) 8.60.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 89.08 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE.
Cool to 45-50C. Mix well and pour into sterile Petri plates.