Ready Pierce C18 Spin Column 89870 Thermo Scientific

Pierce C18 Spin Columns (Protein sample preparation)
89870 Pierce C18 Spin Columns, 25 spin columns each containing 8mg of C18 resin
Introduction
Peptide samples can be purified and concentrated for a variety of applications using the Thermo Scientific Pierce C18 Spin
Columns. Each spin column contains a porous C18 reversed-phase resin with excellent binding and recovery characteristics
at a wide range of peptide concentrations. The spin column format allows simultaneous processing of multiple samples
without the need for laborious repeat pipetting.
Matrix-assisted laser desorption ionization (MALDI-) and electrospray ionization (ESI-) mass spectrometry (MS) are vital
tools for the study of biological compounds because of the high sensitivity and mass accuracy. MS methods are commonly
used for examination of post-translational modifications and identification of proteins by peptide mapping. However, many
of the buffers and compounds common to biological samples interfere with both MALDI-MS and ESI-MS. Pierce C18 Spin
Columns remove interfering contaminants and release peptides in MS-compatible solutions, resulting in increased sensitivity
and a high-quality spectrum. Although Pierce C18 Spin Columns are designed primarily for MS applications, they may be
used for other applications such as peptide concentration and clean-up for peptide sequencing.
Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) provide high sensitivity and mass accuracy; however, interfering molecules from the sample can result in reduced sensitivity and poor-quality spectra. Sample clean-up with C18 resins significantly improves signal-to-noise ratios and sequence coverage by removing MS-incompatible salts and detergents commonly used in protein or peptide preparation. The optimized protocol for the Pierce C-18 Spin Columns effectively removes contaminants and elutes peptides in MS-compatible solutions.
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