Potato Dextrose Agar 500 Gram Himedia

Potato Dextrose Agar 500 Gram Himedia
Media Mikrobiologi Potato Dextrose Agar adalah media general yang di rekomendasikan untuk isolasi dan penghitungan jumlah kapang&khamir
Kemasan / Berat netto : 500 gram
Potato Dextrose Agar M096
Potato Dextrose agar is recommended for the isolation and enumeration of yeasts and moulds from dairy and other food products.
Composition**
Ingredients Gms / Litre
Potatoes, infusion from 200.000
Dextrose 20.000
Agar 15.000
Final pH ( at 25C) 5.60.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 39 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1
ml. Do not heat the medium after addition of the acid.
Principle And Interpretation
Potato Dextrose Agar is recommended by APHA (1) and F.D.A. (2) for plate counts of yeasts and moulds in the examination
of foods and dairy products (3). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures
of certain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production (4).
It is also recommended by USP (5), BP (6) ,EP (7) and JP (8) for growth of fungi. Potato infusion and dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits
the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render
the agar unable to solidify.