Plate Count Agar (PCA) 500 gr | Oxoid CM0325B

Plate Count Agar (PCA) 500 gr | Oxoid CM0325B
Plate Count Agar 500 gr | Oxoid CM0325B
Kemasan : 500 gram
a medium for the enumeration of viable organisms in milk and dairy products
Typical Formula*
gm/litre
Tryptone
5.0
Yeast extract
2.5
Glucose
1.0
Agar
9.0
pH 7.0 0.2 @ 25C
* Adjusted as required to meet performance standards
Directions
Add 17.5g to 1 litre of distilled water. Dissolve by bringing to the boil with frequent stirring, mix and distribute into final containers. Sterilise by autoclaving at 121C for 15 minutes.
Description
Plate Count Agar is equivalent to the medium recommended by the APHA1and the PHLS 2 for the plate count of micro-organisms in food, milk and other dairy products.
The APHA method consists of the preparation of pour plates using diluted samples, and counting colonies after incubation. Incubation is for 48 hours at 32C or at 35C for theStandard Plate Count. For the enumeration of micro-organisms with other temperature requirements, plates may also be incubated for 7-10 days at 5-7C: for 3-5 days at 20C; for 2-3 days at 45C; or for 48 hours at 55C. See the APHA1 publication for details.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30C and use before the expiry date on the label.
Store the prepared plates at 2-8C.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel