Media Mikrobiology Tryptose blood agar base 500gr, Oxoid CM0233

Description
A highly nutritious medium specially developed by Casman1,2 for the preparation of a blood agar which will support the growth of many fastidious organisms.
The original formulation included 0.3g dextrose per litre which interfered with the haemolytic reactions. It is now used without dextrose as a standard medium3.
Tryptose Blood Agar Base with added blood gives good haemolytic reactions and without blood it will sustain good to excellent growth of many demanding organisms. However, to improve the growth of certain organisms e.g. Neisseria meningitidis and Streptococcus pneumoniae, the addition of 1g of yeast extract (LP0021) to one litre of Tryptose Blood Agar Base can be made.
Technique
Lightly inoculate the surface of the medium and incubate aerobically at 35°C for 18-24 hours in 5-10% carbon dioxide (if necessary) or incubate anaerobically at 35°C for 48 hours to enhance haemolysis and to restrict unwanted commensal growth.
Carry out microscopy and other identification tests on the isolated colonies.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel