MYP AGAR 500g Oxoid - CM0929

MYP Agar is a selective and differential medium developed by Mossel et al.4. The diagnostic features of the medium rely upon the failure of Bacillus cereus to utilise mannitol and the ability of most strains to produce phospholipase C. The medium is made selective by the addition of Polymyxin B which will inhibit Gram-negative bacteria. MYP Agar has proved to be very effective for detecting B. cereus even for ratios as challenging as one cell of Bacillus cereus to 106 cells of other organisms4.
Technique
Dry the surface of the agar medium. Prepare the food sample by making appropriate dilutions in Peptone Water CM0009.
Spread 0.1ml of these dilutions over the surface of the agar plate using a sterile glass spreader.
Incubate at 30° C for 18-40 hours.
Count the number of typical colonies and calculate the viable count.
Typical colonies of Bacillus cereus are rough and dry with a bright pink background surrounded by an egg yolk precipitate.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Polymyxin B Supplement SR0099 should be stored at 2-8° C.
Store the prepared plates at 2-8°C.